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Immunoblot Analysis with Anti-HC Antibody.
Proteins were separated by SDS-PAGE using a 15% gel. At left (lanes 1 to 4) are protein bands visualized by silver staining; at right (lanes 5 to 8) are immunoblots analyzed with anti-HC antibody. Lanes 1 and 5, whole cells; lanes 2 and 6, isolated chloroplasts; lanes 3 and 7, isolated cp-nucleoids; lanes 4 and 8, DNA-cellulose 1 M NaCl eluate; double arrowhead indicates the HC protein.
