Figure 6.

Cellular Immunolocalization of HC with Anti-HC Antibody.

(A) and (B) Fluorescence immunolocalization of HC to cp-nucleoids.

(A) DAPI staining of the same cells shown in (B).

(B) HC localization with a fluorescein isothiocyanate–labeled secondary antibody.

A dividing cell is shown in the inset. Yellow indicates the localization of HC. cpn, chloroplast nucleoids; n, nucleus. Bar in (B) = 1 μm for (A) and (B).

(C) and (D) HC localization to cp-nucleoids by immunoelectron microscopy. Cells are shown at two different magnifications after staining with anti-HupA antibodies and gold-labeled secondary antibodies. Gold particles are present at the cp-nucleoid. cp, chloroplast; mt, mitochondrion; n, nucleus. Bars = 0.5 μm.