Figure 7.

Time-Course Study of Cellular HC Level.

The cells were synchronized by a 12-h-light/12-h-dark cycle at 45°C. cpDNA replicated during the second light period, and most chloroplasts divided in the middle of the second dark period. Top and middle gels show RNA gel blot analysis using CmhupA probe. rRNA bands were visualized by methylene blue staining. Total RNA (5 μg/lane) was used. Bottom gel shows immunoblot analysis using anti-HC antibody. Whole cell lysates (10 μg protein/lane) were separated by SDS-PAGE on 15% gels.