Table 2.
Germination and Growth of Seedlings of Line L10 at 28°C
| Wild Type
|
Mutant
|
||||
|---|---|---|---|---|---|
| Time Span (h) | Organ | Increase in Length (cm) | Viability (%) | Increase in Length (cm) | Viability (%) |
| 1 to 168 | Shoots | 7.4 ± 2.5 | 100 | 7.1 ± 2.0 | 100 |
| Roots | 19.7 ± 5.2a | 100 | 23.8 ± 5.8a | 100 | |
| 24 to 168 | Shoots | 7.1 ± 2.0 | 100 | 7.5 ± 2.1 | 100 |
| Roots | 18.3 ± 5.5b | 100 | 23.0 ± 6.1b | 100 | |
| 48 to 168 | Shoots | 7.4 ± 2.1c | 100 | 5.6 ± 1.6c | 100 |
| Roots | 17.9 ± 4.9 | 100 | 19.0 ± 5.9 | 100 | |
| 72 to 168 | Shoots | 4.8 ± 1.9 | 100 | 4.7 ± 1.8 | 100 |
| Roots | 9.0 ± 5.7 | 100 | 8.1 ± 7.6 | 100 | |
Both wild-type and homozygous hsp101-m5::Mu1 kernels (L10) were allowed to imbibe for 1, 24, 48, or 72 h at 28°C in 0.1 mM CaCl2. After imbibition, kernels or seedlings were further incubated at 28°C until day 7 (168 h). Growth of both shoot and roots between the end of imbibition and day 7 was estimated as increase in length and percent viability. Data for growth are means ± sd.
Significantly different according to two-tailed t test (P < 0.005). All other pairs are not significantly different (P = 0.05). Combined data from three experiments.
Significantly different according to two-tailed t test (P < 0.002). All other pairs are not significantly different (P = 0.05). Combined data from three experiments.
Significantly different according to two-tailed t test (P < 0.001). All other pairs are not significantly different (P = 0.05). Combined data from three experiments.