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SKU5 Localizes to Microsomal Membranes, Supernatant, and Cell Wall Fluid Fractions.
Protein gel blot probed first with anti-SKU5 antibody (A) and then with an anti-nitrilase antibody (B) (cytoplasmic control; 38 kD). Lane 1, wild-type supernatant from the 100,000g spin; lane 2, wild-type pellet from the 100,000g spin; lane 3, wild-type cell wall fluid obtained from a low-speed spin of intact seedlings; lane 4, same as lane 3 except that the extraction buffer contained 1 M NaCl.
