Table 1.
Growth Measurements of Wild-Type and sku5 Organs under Various Conditions
| Roots on Tilted 1.5% Agar |
Roots on Vertical 0.8% Agar |
Roots in 0.8% Agar |
Roots in Liquid GM |
Etiolated Hypocotyls in Air |
|
|---|---|---|---|---|---|
| WTa lengthb | 23.9 ± 2.5 | 25.1 ± 2.8 | 24.0 ± 3.2 | NDc | 14.8 ± 1.1 |
| sku5 lengthb | 21.5 ± 2.4 | 21.0 ± 2.5 | 20.7 ± 2.8 | ND | 13.3 ± 1.0 |
| P value | 6.3 × 10−7 | 1.9 × 10−14 | 2.7 × 10−48 | NAd | 5.3 × 10−29 |
| WT anglee | 14.2° ± 4.8 | 2.8° ± 8.0 | 0.14° ± 4.9 | NA | NA |
| sku5 anglee | 59.4° ± 7.0 | 31.7° ± 11.3 | 0.55° ± 5.6 | NA | NA |
| P value | 5.9 × 10−97 | 1.5 × 10−38 | 0.47 | NA | NA |
| WT CFRf | ND | 2.8 ± 4.1 | 2.9 ± 3.1 | 2.6 ± 2.6 | NA |
| sku5 CFRf | ND | 17.1 ± 4.9 | 16.7 ± 5.2 | 13.2 ± 5.2 | NA |
| P value | NA | 3.3 × 10−30 | 1.2 × 10−31 | 2.5 × 10−24 | NA |
Measurements are averages ± sd; n = sample size; P value = Student's t test probability, where <0.05 is statistically significant. The P values correspond to a comparison between wild-type and sku5 samples of a given treatment. 47<n<93 for all samples except “Roots in 0.8% Agar” wild type and sku5 length (418<n<422) and “Etiolated Hypocotyls in Air” wild type and sku5 length (145<n<164).
WT, Wild type.
Average organ length in millimeters.
ND, Not determined.
NA, Not applicable.
Average angle that roots grew, where 0° is vertical and positive numbers were to the left of vertical, as viewed from above the agar surface.
The number of epidermal cell files that crossed a 1-mm-long line drawn down the longitudinal axis of the root, from ∼1.5–2.5 mm from the tip. CFR, Cell file rotation.