FIG. 1.

Targeted inactivation of the MRP-14 gene. (A) The murine MRP-14 gene consists of three exons (grey bars). Disruption of the MRP-14 gene was achieved by insertion of the lacZ-neo cassette into exon 2 immediately following the transcriptional start site. BH, BamHI; B, BglII; EI, EcoRI; S, SacI; X, XbaI. (B) Southern hybridization analysis of mouse tail DNA digested with BglII and hybridized with probe E. (C) Ethidium bromide-stained 1.8% agarose gel of PCR products generated from amplified mouse tail DNA.