Figure 3.

nhx1Δ cells have a similar morphological phenotype to vps27Δ cells. Immunofluorescence was performed as described in MATERIALS AND METHODS in wild-type (WT; SF838–9Dα), nhx1Δ (KEBY15), and vps27Δ cells (KEBY12), by using monoclonal antibodies to ALP or Pep12p, and polyclonal antibodies to Vps10p, Vph1p, or Vma2p. Staining for Vps10p, Pep12p, and ALP was enhanced by using secondary anti-mouse or anti-rabbit biotin-conjugated antibodies followed by a tertiary streptavidin-FITC conjugated antibody. Vph1p and Vma2p were detected by using a secondary Alexa-conjugated anti-rabbit antibody.