Unlike nhx1Δ or
nhx1Δ vma2Δ cells,
vma2Δ cells do not show a class E Vps−
morphological phenotype. Immunofluorescence was performed as described
in Figure 4, with wild-type (WT: SF8389Dα), nhx1Δ
(KEBY15), vma2Δ (KEBY26), and nhx1Δ
vma2Δ (KEBY34) cells and anti-Pep12p and anti-Vph1p
antibodies. Images were captured by using a fluorescence microscope
fitted with a digital camera.