Table 1.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pKEB24 | NHX1 in pBluescript II KS+ | This study |
| pKEB32 | nhx1Δ∷TRP∷TRP1 construct in pBluescript II KS+ | This study |
| pKEB35 | 123 bp triple HA epitope tag sequence in pCR-Blunt | This study |
| pKEB36 | CEN-URA3 plasmid with NHX1 gene (pKEB37) with ATG of YDR455C mutated to ATT | This study |
| pKEB37 | CEN-URA3 plasmid with NHX1 gene | This study |
| pKEB38 | pKEB36 with a triple HA epitope tag inserted in-frame immediately before the stop codon of NHX1 | This study |
| pKEB39 | Integrating plasmid for NHX1-HA allele (loop in/loop out) | This study |
| pKEB43 | nhx1Δ∷Kanr construct in pBluescript II KS+ | This study |
| pKEB44 | CEN-URA3 plasmid with NHX1-HA D201N | This study |
| pKEB45 | CEN-URA3 plasmid with NHX1-HA E225Q | This study |
| pKEB46 | CEN-URA3 plasmid with NHX1-HA D230N | This study |
| pKEB47 | CEN-URA3 plasmid with NHX1-HA E355Q | This study |
| pKEB53 | CEN-LEU2 plasmid with NHX1-HA | This study |
| pKEB58 | CEN-LEU2 plasmid containing PEP4 | This study |
| pFP1 | CEN-URA3 plasmid with NHX1-HA D201N E225Q | This study |
| pFP2 | CEN-URA3 plasmid with NHX1-HA D201N D230N | This study |
| pBL2 | GEF1 in pBluescript II KS+ | This study |
| pBL20 | gef1Δ∷LEU2 construct in pBluescript II KS+ | This study |
| pKH105 | pQE16 (Qiagen, Valencia, CA) with four copies of the triple HA epitope | This study |
| pKJH2 | vps27Δ∷LEU2 construct | Piper et al. (1995) |
| pSN55 | CEN-URA3 plasmid encoding A-ALP | Nothwehr et al. (1993) |
| pCY40 | vma2Δ∷LEU2 construct | Yamashiro et al. (1990) |
| pMK10 | vma3Δ∷URA3 construct | Kane et al. (1992) |
| pTS18 | CEN-URA3 plasmid containing PEP4 | Ammerer et al. (1986) |
| pJLU34 | CEN-URA3 plasmid encoding Ste3p-GFP | Urbanowski and Piper (1999) |
| pSL1417 | Integrating plasmid for PEP4 (loop in/loop out) | Dr. G. F. Sprague, Jr. |