TABLE 3.
Up-regulated genes in both MCF+FIR and MCF+SOD cells
| GenBank no. | Gene name | Functiona | Foldb change in:
|
|
|---|---|---|---|---|
| MCF+FIR/controlc | MCF+SOD/MCF-7c | |||
| L25081 | Transforming protein RHOC | Apoptosis, proliferation, carcinogenesis | 5.8 | 3.2 |
| M86400 | 14-3-3 zeta | Apoptosis | 5.1 | 3.2 |
| M14745 | BCL-2-alpha | Apoptosis | 3.1 | 3.3 |
| Q99537 | BRCA1, RHO7, and VATI | Apoptosis | 2.2 | 3.4 |
| AI376462 | Lupus KU autoantigen protein P86 | Apoptosis, radioresistance | 2.0 | 6.4 |
| V00568 | MYC proto-oncogene protein | Antiapoptosis | 5.5 | 3.3 |
| S40706 | GADD 153 (growth arrest and DNA-damage-inducible protein) | DNA damage response | 5.4 | 9.1 |
| Cell cycle control | ||||
| U09579 | p21 (waf1, CDKNIA) | Cell cycle regulator | 4.6 | 5.5 |
| M25753 | Cyclin B1 | Cell cycle control | 3.3 | 2.1 |
| X51688 | Cyclin A | Cell cycle control | 3.3 | 6.4 |
| X12530 | B-lymphocyte antigen CD20 | Cell cycle antigen | 3.5 | 7.7 |
| Y00371 | Heat-shock cognate 71-kDa protein | Stress response | 8.3 | 3.6 |
| M14091 | Thyroxin-binding globulin precursor | Stress response | 3.4 | 2.5 |
| L38503 | Glutathione S-transferase theta 2 | Stress response | 3.2 | 6.2 |
| Q64347 | Zinc finger protein 101 | Transcription activation | 4.2 | 2.1 |
| X66899 | RNA-binding protein EWS | Transcription activation | 3.4 | 5.5 |
| G29507 | General transcription factor | Transcription factor | 4.1 | 3.8 |
| M31523 | Transcription factor 3 | Transcription factor | 3.2 | 6.3 |
| Q00421 | GA-binding protein beta-2 chain | Transcription factor | 3.2 | 12.2 |
| M55422 | Krueppel-related zinc finger protein | Nuclear receptors | 8.3 | 5.6 |
| S72008 | CDC10 protein homolog | Growth regulator | 4.4 | 6.2 |
| M63488 | Replication protein A | DNA replication | 4.4 | 8.8 |
| J04088 | DNA topoisomerase II alpha isozyme | DNA replication | 4.4 | 8.5 |
| X06745 | RNA1 DNA polymerase alpha | DNA synthesis | 3.9 | 4.2 |
| U57342 | Myeloid leukemia factor 2 | Cell differentiation | 18.9 | 8.9 |
| M27396 | Asparagine synthetase | Cell proliferation | 6.3 | 7.3 |
| AA629900 | NAD-dependent methylenetetrahydrofolate dehydrogenase | Cell proliferation | 5.3 | 3.7 |
| H38136 | TGF-βd-inducible early growth response 2 | Cell proliferation | 3.2 | 4.5 |
| D13748 | Eukaryotic initiation factor 4A-I | Cell proliferation | 4.1 | 7.7 |
| L07594 | TGF-β receptor type III precursor | Cell proliferation, differentiation | 3.3 | 7.4 |
| X60221 | ATP synthase B chain, mitochondrial precursor | ATP synthesis | 3.2 | 6.4 |
| AF026816 | Putative oncogene protein | Oncogene | 12.4 | 6.4 |
a
Gene function was updated by MedLine database searches.
b
Results represent the quantitation of one hybridization of two sets of fluorescence-labeled RNA probes, not including values with changes of less than twofold.
c
MCF+FIR profiles were obtained by normalizing gene expression levels of MCF+FIR radioresistant clone 8 to the level of the revertant MCF+FIR clone 10; MCF+SOD profiles were obtained by normalizing expression levels of the MCF+SOD cell line to the level of wild-type MCF-7 cells.
d
TGF-β, transforming growth factor β.