Figure 5.

Effect of anti-TGF-β antibody on the expression of the endothelial cell markers ICAM-2 (A) and vWF (B) in the presence of anti-TGF-β and on formation of vessel-like structures in EBs (C and H). EBs grown in the presence or absence of monoclonal anti-TGF-β antibody (15 μg/ml) were analyzed for ICAM-2 (A) and vWF (B) expression by RT-PCR. The values obtained by densitometry of the bands are presented as percentages of the values obtained for the housekeeping gene β-actin. The values represent the averages obtained from three gels. Nine-day-old EBs grown in the presence (F–H) or in the absence (C–E) of anti-TGFβ antibody were analyzed by light microscopy (C and F) and by indirect immunofluorescence (D, E, G, and H) with ICAM-2 antibody to detect endothelial cells forming vessel-like structures. Magnifications, ×200 (C, D, F, and G); ×630 (E and H). Bars, 50 μm (C, D, F, and G; 10 μm (E and H). The outlined boxes in D and G indicate the fields illustrated at higher magnification in E and H.