FIG. 1.

SREBP1a interacts with the coactivator p300. Cos7 cells were transfected with EGFP-SREBP1a and HA-tagged p300, either alone or together, and the subcellular localization of the transfected proteins was determined as described in Materials and Methods. (A to C) p300 (red) is localized to nuclear speckles in cells transfected with p300-HA alone. (D to F) EGFP-SREBP1a (green) shows a uniform nuclear staining in the absence of p300 expression. (G to I) EGFP-SREBP1a (green) is recruited into nuclear speckles when coexpressed with p300-HA (red).