Table 2.
The PHO regulated genesa
| ORF | Gene | No. of Pho4 binding site on the
promoter
|
Descriptionb | |
|---|---|---|---|---|
| CACGTG | CACGTT | |||
| Phosphatases | ||||
| YBR093c | PHO5 | 1 | 1 | Repressible acid phosphatase (p60) |
| YAR071w | PHO11 | 1 | 1 | Repressible acid phosphatase (p56) |
| YHR215w | PHO12 | 1 | 1 | Repressible acid phosphatase (p58) |
| YDR481c | PHO8 | 1 | 1 | Repressible vacuolar alkaline phosphatase |
| Pi transporter | ||||
| YML123c | PHO84 | 2 | 2 | High-affinity H+/Pi cotransporter |
| YBR296c | PHO89 | 1 | 1 | Na+/Pi cotransporter |
| YJL117w | PHO86 | 1 | 1 | Involved in Pi transport |
| PHO81 family | ||||
| YGR233c | PHO81 | 1 | 0 | Cyclin-dependent kinase inhibitor |
| YPL110c | 1 | 1 | PHO81 homolog | |
| YHR136c | SPL2 | 4 | 0 | PHO81 (ankyrin) homolog |
| YFL004w family | ||||
| YFL004w | PHM1c/VTC2e | 1 | 0 | Involved in polyP synthesisf |
| YPL019c | PHM2c/VTC3e | 2 | 1 | Homology to YFL004w |
| Involved in polyP synthesisf | ||||
| YJL012c | PHM3c/VTC4e | 2 | 0 | Homology to YFL004w |
| Involved in polyP synthesisf | ||||
| YER072w | PHM4c/VTC1e/NRF1e | 1 | 1d | Homology to YFL004w |
| Involved in polyP synthesisf | ||||
| Vacuolar transporter chaperone | ||||
| Others | ||||
| YER062c | HOR2/GPP2 | 1 | 0 | DL-glycerol phosphatase |
| YPL018w | CTF19 | 2 | 1 | Important for chromosome segregation |
| YER055c | HIS1 | 1 | 0 | ATP phosphoribosyltransferase |
| YDR452w | PHM5c | 1d | 0 | Similar to acid sphingomyelinase |
| Supposed vacuolar polyphosphatasef | ||||
| YDR281c | PHM6c | 1 | 2 | Hypothetical protein |
| YOL084w | PHM7c | 1d | 0 | Similarity to S. pombe SPAC24H6.13 and YMR266w |
| YER037w | PHM8c | 0 | 1 | Similarity to hypothetical protein YGL224c |
| YER038c | 0 | 0 | Function unknown | |
a
The genes listed in this table showed more than two-fold derepression ratio in more than five independent experiments out of the eight in Figure 2 except for YGR233c (PHO81) and YPL110c.
b
Description taken from Yeast Proteome Database http://www.proteome.com/.
c
PHM1-8 are novel genes, named in this study.
d
5/6 nucleotide sequences match to the Pho4 binding sequences.
e
VTC1–VTC4 and NRF1 were independently named and characterized by Cohen et al., (1999) and Murray and Johnson (2000), respectively, in course of this study.
f
These functions were identified in this study.