FIG. 6.

Validation of mRNAs displaying either preferential or nonpreferential polysomal association in a pVHL-dependent fashion. (A) Abundance of mRNAs encoding VEGF and cyclin D1, which preferentially associated with polysomes in pVHL-deficient cells, was assessed by Northern blotting and quantitated as described in the legend for Fig. 5. (B) Abundance of Glut-1 mRNA, which is not preferentially associated with polysomes in pVHL-deficient cells, was assessed as described in the legend for Fig. 5. β-Actin mRNA signals served to both validate the results in Fig. 5 and monitor the evenness in loading and transfer of samples.