TABLE 1.
pVHL can associate with translation regulatory factors in yeasta
| Candidate pVHL-interacting proteins | GenBank accession no. | Function | No. of clones |
|---|---|---|---|
| Translation initiation factor 3 subunit 4 (eIF3) | NP003746 | Translational regulation | 1 |
| Translation elongation factor 1-alpha (EF-1α) (EF-TU) | AAA52367 | Translational regulation | 4 |
| Glucose-regulated protein (GRP 78) | H56456S1 | Molecular chaperone | 1 |
| Heat shock 60-kDa protein 1 (HSP 60) | P10809 | Molecular chaperone | 1 |
| Heat shock protein 90 (HSP 90) | HHHU86 | Molecular chaperone | 2 |
| VHL-binding protein 1 (VBP-1) | Q15765 | Cytoplasmic export of pVHL | 1 |
| 26S protease regulatory subunit (Tat-binding protein 1) | P17980 | Protein degradation | 1 |
| Gelsolin | NP000168 | Other | 1 |
| Metallothionein 2A | H56373S3 | Other | 1 |
| Filamin (actin-binding protein 280) ABP-280 | NM001456 | Other | 1 |
| RIE 2 sid 2705 | BAA84708 | Other | 1 |
| CGI-111 protein | AAD34106 | Other | 1 |
| Antioxidant enzyme BP 66 | AAF03750 | Other | 1 |
| Gene SA protein | I54401 | Other | 1 |
| X-Pro dipeptidase | L10320 | Other | 2 |
a
Shown are the results of a yeast two-hybrid survey leading to the identification of potential pVHL-interacting proteins from a human kidney cDNA library. Some of the clones were represented several times (number of clones, right column). The interaction of pVHL with all of the gene products listed was confirmed by yeast mating (explained in Materials and Methods).