Figure 1.

Schematic diagram of lamin expression constructs. Xenopus lamin B₁ and human lamin A cDNAs were used to construct mutants. All constructs were tagged with GFP. Three of these proteins (wt, Δ2⁺, and Δ3⁺) were also constructed as fusions with the 11 amino acid HA tag, giving identical results. A summary of the results obtained by transfection of each mutant construct is shown to the right. The number of aggregates per cell was counted directly and shown is the mean ± SEM for two experiments in which 100 cells each were counted. N/A, not applicable. N.D., not determined.