Figure 5.

Effect of lamin mutants on the distribution of CHO PCNA. CHO cells were transfected with GFP-lamins as in Figure 2. After 48 h, cells were either fixed immediately (A) or first extracted with Triton X-100 before fixation (B). Cells were then stained with antibody against PCNA as described in MATERIALS AND METHODS. Δ3⁺, Δ3⁺-CaaX, and LA-head gave results identical to WTLB1, LB1-CaaX, and LA-CaaX, respectively.