Figure 3.

Further Evidence for 3a MP:GFP Traffic out of the SE-CC Complex.

(A) and (B) CoYMV:3a:GFP transgenic tobacco. 3a MP:GFP mRNA is localized in CCs but not in any other cells by in situ hybridization using antisense 3a MP RNA probes.

(A) External phloem of a petiole.

(B) Internal phloem of a stem.

(C) and (D) Nontransgenic tobacco. In situ hybridization using antisense 3a MP RNA probes produced no hybridization signals.

(C) External phloem of a petiole.

(D) Internal phloem of a stem. X, xylem.

(E) Diagram of a graft union consisting of a CoYMV:3a MP:GFP transgenic stock and a nontransgenic scion.

(F) Transverse view of the internal phloem of a nontransgenic scion stem. Bright-field images were taken in grayscale and pseudocolored in red. 3a MP:GFP is present in pith cells (arrowheads) as well as in the phloem (Ph).

Bars in (A) and (C) = 20 μm; bars in (B), (D), and (F) = 10 μm.