Figure 6.
Effect of FTI on subcellular localization and posttranslational processing of ras proteins. (A) Pan-ras immunoreactivity in Panc-1 cells treated with 0.1% DMSO for 72 hours. Note predominant staining of cytoplasmic membrane. (B) Pan-ras immunoreactivity in Panc-1 cells treated with 10 µM L-744,832 for 72 hours. Increased cytoplasmic staining is noted. (C) Immunoblot analysis of FTI-induced changes in posttranslational processing of Ras proteins. Cells were treated with 0.1% DMSO versus 10 µM L-744,832 for 72 hours. “U” indicates unprocessed upper band. “P” indicates processed lower band. Results are representative of two independent experiments.