Figure 2.

RBX1a Is a Component of the SCF Complex in Arabidopsis.

(A) Recombinant GST-RBX1a or GST was added to extracts prepared from Arabidopsis seedlings. The GST pulldowns were examined for the presence of CUL1 by protein gel blot analysis using α-CUL1 antibody. The two CUL1 species are unmodified and RUB-modified protein. The three lanes are from the same autoradiogram.

(B) As in (A) except that extract was prepared from a tir1 line expressing TIR1-myc. CUL1, TIR1-myc, and GST-RBX1a were detected in the pulldown by protein gel blot analysis. The left lane is a control pulldown using GST alone.