Figure 3.

Visualization of Cortical MTs in Wild-Type Root Epidermal Cells.

Roots of 3-day-old seedlings were used for MT labeling with a monoclonal antibody against α-tubulin. Antibody-labeled MTs were detected with fluorescein isothiocyanate–conjugated secondary antibodies, and the signals of fluorescent MTs (green) were visualized using a confocal microscope. Double-headed arrows indicate the elongation axis.

(A) View of a whole root tip showing root cap cells (asterisk), early-elongating cells (arrow), and rapidly elongating cells (arrowhead).

(B) Rapidly elongating cells showing the transverse orientation of cortical MTs.

(C) Early-elongating cells showing the transverse orientation of dense cortical MTs.

Bar in (A) = 40 μm; bars in (B) and (C) = 8 μm.