Figure 9.

Redox Activation State and Dimerization Degree of AGPase in Transgenic Tubers Expressing a Heterologous Suc Phosphorylase in the Cytosol.

Tubers of 8-week-old wild-type plants and three independent transgenic lines were analyzed for Suc content (A), starch (B), hexose phosphates (C), overall AGPase activity when DTT was included in the assay (Vred) (D), redox activation state of AGPase (Vsel/Vred) (E), and dimerization of AGPB protein in nonreductive SDS-PAGE using the maize brittle-2 antibody (F). Each lane contains the proteins originating from 1 mg fresh weight. Results are means ± se; n = 3 tubers from different plants, except for (F), which documents a representative example. FW, fresh weight.