Table 2.
Estimated in Vivo Mass-Action Ratios of Enzyme and Transport Reactions Involved in Suc-to-Starch Conversion
Molar Mass-action Ratio
|
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---|---|---|---|---|
Reaction | Formula | Attached Tubers | Detached Tubers | Theoretical Equilibrium Constant (Keq) |
SuSy (cytosol) |
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0.181 | 0.632 | 0.15 to 0.56a |
UGPase (cytosol) |
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1.48 | 3.21 | 3.2b |
Fructokinase (cytosol) |
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0.032 | 0.085 | 851c |
Phosphoglucoisomerase (cytosol) |
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3.47 | 4.05 | 2c |
PGM (cytosol) |
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11.9 | 10.5 | 19c |
Glc-6-P/Pi translocator |
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2.04 | 2.97 | 1d |
3PGA/Pi translocator |
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1.85 | 3.31 | 1d |
Adenylate translocator |
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0.359 | 0.961 | 1d |
PGM (plastid) |
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0.046 | 0.078 | 0.053c |
AGPase (plastid) |
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1.6 × 10−2 | 4 × 10−4 | 1e |
Inorganic pyrophosphatase (plastid) |
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0.323 | 0.373 | 1000b |
The data from Table 1 were used to calculate the in vivo molar mass-action ratios (ratio of product to substrate concentration) for each reaction. The theoretical equilibrium constant (ratio of product/substrate concentration) at which the net flux is zero) of each reaction is shown for comparison.
Estimated value.