Figure 6.

GFA2::GUS Expression in the Wild Type, GFA2-GFP Protein Localization in the Wild Type, and FIE::FIE-sGFP Expression in the gfa2 Mutant.

(A) and (B) Histochemical assay for the expression of the GFA2::GUS transgene in the whole plant (A) and the flower (B). Longer histochemical staining showed expression in the petals.

(C) Trichome from a transgenic plant expressing 35S::GFP.

(D) Trichome from a transgenic plant expressing 35S::GFA2-GFP.

(E) to (G) Mitotracker-stained root hair from a transgenic plant expressing 35S::GFA2-GFP.

(E) Mitotracker fluorescence.

(F) GFP fluorescence.

(G) Merger of red and green channel fluorescence.

(H) and (I) Expression of the FIE::FIE-GFP construct in the wild type (H) and the gfa2 mutant (I).

pn, polar nucleus; sn, secondary nucleus.