Figure 1.

Transient Transformation with RNAi-Generating Gene Constructs Interferes with the Expression of Target Genes.

(A) Schemes of gene constructs. Different box shades indicate regions of the respective reporter genes used to create each effector construct. Arrowheads indicate the orientation of each gene or gene fragment. Numbers below the boxes represent the size (in bp) of each segment or entire gene (not drawn to scale).

(B) Reporter construct Ubi1-GUS and internal transformation control Ubi1-LUC were cobombarded with either the Ubi1-Empty vector (−) or the effector construct Ubi1-GUS(RNAi).

(C) Reporter construct Ubi1-LUC and internal control Ubi1-GUS were cotransformed with either the Ubi1-Empty vector (−) or the effector construct Ubi1-LUC(RNAi).

For (B) and (C), all plasmids were mixed at a 1:1:1 ratio (reporter:effector:internal control). Closed and open bars represent GUS and LUC activities, respectively, ± se (n = 6).