Figure 1.

Map of the AC29 Locus.

The probes acB and acC are shown. The approximate location of the point mutations, insertions (wedges), and deletions (−) of the acy mutants are marked. The exons and introns of the Alb3 gene are indicated with black bars and lines, respectively. Untranslated regions are shown as open bars. Transcription proceeds from left to right. The primers used are indicated. Plasmids and phages used in transformations are shown at bottom. Rescue of the mutant phenotype is indicated by +. Restriction sites are indicated at top: Bg, BglII; Bs, BstEII; N, NsiI; R, EcoRI; Sc, SacI; Sf, SfiI; Sm, SmaI; Sp, SpeI; Xb, XbaI.