Figure 2.

DNA Gel Blot Analysis of ac29 Mutants.

DNA was isolated from the diploid parent (nic7 ac29-2 mt⁻ pf14/ ++ mt⁺ +) and the diploid acy9, acy10, acy20, acy25, and acy32 mutant strains and from the haploid ac29-3 mutant containing the mt⁺ chromosome from acy32. The DNAs of the blots were digested with BglII (A) or SacI ([B] and [C]) and probed with the acB probe ([A] and [B]) or the acC probe (C).