Figure 8.

The Alb3-HA Protein Is Associated with Two Complexes.

Thylakoid membranes from the T2 transformant were solubilized with dodecyl maltoside and fractionated on a Suc density gradient. Fractions of 1 mL were collected, and 32-μL aliquots were used for PAGE and immunoblotting. The antibodies used are indicated at left. Antibodies specific for LHCII, PSII (PsbB), and PSI (PsaE) were used. Masses are indicated in kD and were determined by comparison with molecular mass standards provided with a molecular marker kit (Sigma). HA, HA-tagged Alb3 protein.