Skip to main content
. 2000 Dec;11(12):4359–4368. doi: 10.1091/mbc.11.12.4359

Figure 2.

Figure 2

The mutant alleles I870T, I870R, and Q975R/D999V respond in vivo to the activation of PKC but not to coexpressed RhoAV14. (A) HEK293 cells cotransfected with wild-type or mutant PLD1 alleles and pEF-Bos-HA-RhoAV14 or the empty pEF-Bos-HA vector were assayed in vivo for PLD activity. (B) HEK293 cells overexpressing wild-type and mutant PLD1 alleles were stimulated with 100 nM PMA and were assayed in vivo for PLD activity. Assays were performed over a 30-min time period, and the data shown are representative of three experiments.