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Insensitivity of I870R to increased levels of wild-type RhoA during agonist stimulation. M3 mAChR-expressing HEK293 cells were transfected with pCGN-wild-type PLD1, PLD-K898R, or I870R and pEF-Bos-HA-wild-type RhoA or the empty pEF-Bos-HA vector. After 24 h, a PLD in vivo assay was performed in triplicate in the absence (basal) or presence of 1 mM carbachol. The data are representative of two experiments.
