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Constitutively active ARF1 and ARF6 increase PLD2 activity in HEK 293 cells but not that of PLD1. HEK 293 cells cotransfected with pCGN-hPLD1, mPLD2, or hPLD1-K8989R and the empty pcDNA vector, pcDNA-ARF1 Q71L or pcDNA-ARF6 Q67L (the dominant active alleles of ARF1 and ARF6) were assayed in vivo for PLD activity. The experiments were conducted in triplicate. The values shown are representative of four experiments.
