Figure 1.

Luminescence from Reconstituted Aequorin in Nicotiana plumbaginifolia Seedlings Expressing Aequorin That Has Been Targeted to the Chloroplast Stroma (MAQ 6.3).

Seedlings were in LL (22 μE·m⁻²·s⁻¹) until the time of transfer to DD (white bars along abscissa indicate LL, and black bars indicate DD).

(A) Comparison of dark-stimulated luminescence from MAQ 6.3 seedlings (open circles) and nontransgenic wild-type seedlings (closed circles). Both MAQ 6.3 and wild-type seedlings were incubated in coelenterazine; transition to DD was at 111.7 h after the beginning of LL. There were 10 seedlings per vial, and seedlings were 10 days old.

(B) to (D) Three independent sets of MAQ 6.3 seedlings transferred from LL to DD at 120 h. There were five seedlings per vial, and seedlings were 22 days old. (C) and (D) are redrawn from Johnson et al. (1995).