Figure 5.

Stromal Ca²⁺ Spikes Occur after Lights Off in 24-h Light/Dark Cycles.

(A) and (B) Long-day cycles (LD 16:8).

(C) and (D) Short-day cycles (LD 8:16).

Gray areas indicate dark intervals, and white areas indicate illuminated intervals. Light Intensity was 22 μE·m⁻²·s⁻¹. MAQ 6.3 seedlings were monitored in (A) and (C), whereas nontransgenic wild-type seedlings were monitored in (B) and (D). Both the transgenic and nontransgenic seedlings were treated with coelenterazine. These data are representative traces for two separate experiments for each photoperiod with four to five replicates for each condition.