Figure 7.

Recovery of Fluorescence into Photobleached Golgi Stacks Is ATP and Protein Dependent.

(A) to (C) Golgi stacks in an AtERD2-GFP–transformed epidermal cell. Golgi stacks ([A], circles) were bleached (B), and their fluorescence failed to recover in the presence of ATP depletors (C).

(D) to (F) Golgi bodies ([D], circles) were bleached (E) in a cell treated with the protein cross-linker NEM (20 mM) for 15 min. The fluorescence did not recover under these conditions (F).

Time is expressed in seconds at the bottom left of each frame. Bars = 5 μm.