Figure 6.

Zinc Affects the Abundance of IRT1 mRNA and Protein.

Wild-type (WT [A]) and 35S-IRT1 (line 4) transgenic (B) plants were grown for 2 weeks on B5 plates. Seedlings were transferred subsequently and grown for 3 days on iron-sufficient plates (lane 1), iron-deficient plates (lane 2), iron-sufficient plates supplemented with 500 μM zinc (lane 3), iron-deficient plates supplemented with 100 μM zinc (lane 4), or iron-deficient plates supplemented with 500 μM zinc (lane 5). RNA and protein samples were prepared from each root sample and used to prepare RNA and protein gel blots. The IRT1 cDNA was used to probe the RNA gel blots. Ethidium bromide–stained rRNA is shown as a control for loading. IRT1 protein was detected using the IRT1 affinity-purified peptide antibody.