Figure 2.

Overexpression of TGA2CT in Wild-Type Plants Results in an npr1-Like Phenotype.

(A) Protein gel blot analysis of the TGA2CT protein in 35S::TGA2CT transformants. Protein extracts were made from 2-week-old plants. Independent 35S::TGA2CT transformants (5, 6, 17, and 18) in the wild-type background (35S::TGA2CT [WT]) were compared with untransformed wild-type plants (WT). The His₆-tagged TGA2CT was detected using an antibody against the His tag.

(B) RNA gel blot analysis of PR-1 gene expression. Plants were grown for 2 weeks on MS medium with (+) or without (−) 20 μM INA. Total RNA (10 μg) was used to make the blot, which was probed for the PR-1 and UBQ5 mRNAs.

(C) Disease symptoms caused by P. syringae pv maculicola ES4326. Leaves from 4-week-old wild-type, 35S::TGA2CT (WT) (line 17), and npr1-2 plants were infiltrated with a P. syringae pv maculicola ES4326 suspension of OD₆₀₀ = 0.0001. Photographs were taken of representative leaves 4 days after infection.

(D) Growth of plants on MS medium containing 0.5 mM SA. The photographs were taken 7 days after germination.