Figure 1.

Western blot analysis of PLC-β1 and PLC-γ1. Soluble proteins from whole-mouse oocytes (lanes 2A and 1B) or subcellular fractions (cytosol [lanes 3–4A and 2–3B) and nucleoplasm (lanes 5–6A and 4–5B]) were analyzed by 7.5% SDS-PAGE. Approximately 200 oocytes were pooled for each lane. The gels were electroblotted onto Polyscreen polyvinylidene difluoride membranes and the lanes were probed with either anti-PLC-β1 mAb1 and mAb2 or anti-PLC-γ1 mAb. Mouse brain protein (lane 1A) was used as a positive control. Molecular weights are indicated in kDa. The blots selected for A correspond to oocytes treated without calpain inhibitors; the blots in B corresponded to oocytes treated in presence of calpain inhibitors.