Table 2.

Flow Cytometric Analysis of Colorectal Cancer Cell Lines Treated with 8-Cl-adenosine.

	Sub G1	G1	S	G2	Polyploid
HCT116
0 h	3.61±1.79	38.54±7.00	44.55±2.82	16.91±5.86	-
24 h	5.35±0.79	39.60±1.15	43.28±4.25	17.13±4.40	-
48 h	15.50±1.27*	56.62±4.42	19.00±2.62†	24.38±4.51	-
72 h	15.58±5.91	41.71±1.92	29.12±3.20*	29.17±5.05	+
HCT116-E6
0 h	5.18±1.62	24.07±4.26	43.43±0.64	32.50±4.89	-
24 h	7.33±0.54	26.29±1.85	39.09±3.48	34.62±3.95	-
48 h	15.33±0.54†	24.68±1.72	27.75±2.48f	47.57±2.39	+
72 h	14.75±2.55*	18.25±4.66	35.16±4.09	46.58±2.95	+++
80S14
0 h	10.48±3.73	28.02±3.95	40.37±0.62	31.61±3.60	-
24 h	17.19±4.41	39.02±1.51	36.20±1.79	24.78±1.86	-
48 h	22.45±1.79*	40.64±3.74	28.48±1.76	30.88±5.49	+
72 h	22.73±1.62*	25.39±3.41	28.66±0.92†	45.96±4.32	+++

Each colorectal cancer cell line was treated with 8-Cl-adenosine (5 µM) for the indicated times. Cells were harvested and subjected to flow cytometric analysis as described under Materials and Methods. Data represent the average±SEM of three experiments.

^*p<0.05;

^†p<0.005 when compared to 0-hour sample.