Figure 2.

Results of a rat aortic ring assay for functional angiogenic factors produced by untransfected HME (panel A), HME-β-gal control transfectants (panel B), HME-RhoC transfectants (panel C) and the SUM149 IBC cell line (panel D). Segments of rat aorta were embedded in Matrigel and cultured in the corresponding cell-conditioned media for 4 days and then observed for microvessel outgrowth. Conditioned media from the control cell lines (panels A and B) did not induce microvessel outgrowth. However, conditioned medium from the HME-RhoC transfectants (panel C) produced similar levels of new vessel growth as the SUM149 IBC cell line (panel D).