Figure 6.

IL2 secretion of IgTCR transduced cells after stimulation with immobilized proteins and melanoma cell lines. Unmodified and sFv-ζ modified Jurkat T cells were coincubated with various stimuli, and the supernatants were harvested and tested for IL2 concentration. Stimulators were: UPC, nonspecific antibody; OKT3, anti-CD3 antibody; anti-Id, anti-idiotypic antibody to MB3.6; M21, GD₃+ melanoma cell line; M24, GD₃- cell line. IL2 levels were standardized to OKT3-stimulated secretion for each group (= 100) to control for variation in cell numbers and cell condition, with a range of 400–1200 IU/ml in different assays. The use of no stimulator or bovine serum albumin (BSA) gave no IL2 secretion (not shown) that was equivalent to UPC. The SE was ±20% in quadruplicate assays.