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Mapping of novel transcription factor binding element. (A) Sequences of mutated oligonucleotide used for competition in EMSA analysis for mapping the transcription factor binding sites included in oligo-2. (B) F9 nuclear extracts were incubated with 32P-labeled oligonucleotide without or with a 50-fold excess of unlabeled oligo-1 (−177), oligo-2 (32), or the mutated sequences M1–M8 indicated in the figure, or the homologous mouse oligo-2. Arrowheads indicate specific retarded bands.
