Figure 6.

Transcription activation of wild-type and mutated hTERT core promoter. F9 and HEK cells were transiently cotransfected with pGL-3 construct containing as insert either the wild-type hTERT core promoter or the mutant core promoter sequences M1, M3, or M4, as indicated. Cells were cotransfected with the β-galactosidase expression vector (CMV-β-gal) for calibration of transfection efficiency. Cell extracts were assayed for luciferase activity, which was normalized by the corresponding β-galactosidase value. Data represent normalized relative luciferase fold activity compared with the promoterless pGL3 basic plasmid. Results are means ± SE of two independent experiments, each performed in quadruplicate.