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. 2002 Oct 15;110(8):1123–1132. doi: 10.1172/JCI16029

Figure 4.

Figure 4

Regulation of ROS blocks Bz-423–induced killing. (a) After preincubation of Ramos cells for 30 minutes with FK506 (1 μM, blue), vitamin E (100 μM, red), MnTBAP (100 μM, green), or no inhibitor (black), Bz-423 (10 μM) was added to the cultures. White bars indicate control samples treated with vehicle alone. Using flow cytometry, DHE fluorescence (O2–) was measured at 1 hour, caspase activation at 5 hours, ΔΨm (DiOC6[3]) at 5 hours, and PI permeability and hypodiploid DNA at 24 hours. (b) Effect on cell viability of addition of FK506 at times relative to the addition of Bz-423. The results in a and b represent more than five separate determinations. P < 0.05 for the differences between Bz-423 and control for each inhibitor at each endpoint (Student’s t test).