Figure 6.

Southern blot analysis was carried out on the alveolar lanes 1[(A1), 2(A3), 3(A7)] and the embryonal lanes [4(E3), 5(E4), 6(E5)] tumors by using the MYCN and DDX1 probe. The tumor and normal (thymus) DNA, together with DNA from a neuroblastoma cell line, IMR32 (coamplified with MYCN and DDX1), was digested with EcoR1 and separated on a 1% agarose gel in Tris borate EDTA buffer. After transfer to a nylon membrane, the filter was probed with MYCN, CT_β (a control probe), and DDX1. Coamplification of MYCN and DDX1 is seen in the alveolar tumors in lanes 2 (A3) and 3 (A5) and the control lane 8 (IMR32). Lane 7 is a normal control.