Figure 5.

Effect of JNK on COX-2 mRNA stability after induction with ceramide or CD. RIE-iRas cells were cotransfected with pcDNA3.1/Zeo(+)Luc+3′UTR (0.4 µg) and either an expression plasmid encoding a dominant-negative JNK-1 protein (0.4 µg) or the empty vector (0.4 µg). The transfected cells were then treated with control, ceramide or CD, harvested 12 hours after treatment and assayed for luciferase activity and total protein. Three wells were used for each condition. Data are expressed as means ±SE.