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. 2000 Nov;2(6):540–554. doi: 10.1038/sj.neo.7900107

Figure 2.

Figure 2

Southern blot analysis of the integrated plasmid sequences in HSV-tk- subclones of A3 and B3 that retained some portion of the plasmid sequences. (A) Genomic DNA from A3 and 11 of its HSV-tk- subclones (G1–G39), and (B) genomic DNA from B3 and 12 of its HSV-tk- subclones (G41–G74) was digested with BamHI, which cuts once in the plasmid, and hybridization was performed with the pNCT-Δ probe. The absence of the diffuse band indicates the loss of the telomere, whereas the appearance of a new discrete band indicates the presence of nontelomeric DNA fused on to the end of the chromosome.