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. 2000 Nov;2(6):540–554. doi: 10.1038/sj.neo.7900107

Figure 4.

Figure 4

Nucleotide sequence analysis of the sites of addition of telomeric repeat sequences following the loss of the end of the HSV-tk gene. (A) The nucleotide sequences at the sites of addition of the telomeric repeat sequences on to the end of the HSV-tk gene in subclones G6, G8, and T4 are compared with the sequence of the HSV-tk gene (TK) and telomeric repeat sequence (Tel). Regions of homology (asterisks) and short regions of complementarity between the plasmid and telomeric repeat sequences at the site of addition (bold) are indicated. The sequences of the telomere-specific primers used for PCR are also shown (underlined). (B) The structure of the integrated plasmid sequences in the parental clone B3 compared with the structure of the plasmid sequences in the G6, G8 and T4 subclones with terminal deletions involving the HSV-tk gene. The location of restriction sites for BamHI (Bm), BgIII (Bg) and ClaI (Cl) are shown.