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Induction of cell death in HeLa cells. (A) Cells were exposed to various concentrations of aspirin (ASA) for 24 hours (■) or 48 hours (▲). (B) Cells were exposed to aspirin for the times indicated. Death was assessed by the binding of annexin V to phosphatidylserine exposed on the outside of the apoptotic cell. The percentage annexin V-positive cell was measured using flow cytometry. Mean values±SD are given.
