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Demonstration of IGF2 Apa1 polymorphism with cDNA as template to demonstrate loss of imprinting of cases 5 and 36 and retention of imprinting of cases 35 and 42. Case 6 (which is homozygous for the allele containing the Apa1 site) with DNA as template is included as a control for completion of restriction enzyme digestion; RT+ cDNA made in the presence of reverse transcriptase; RT- control cDNA reaction in which reverse transcriptase is omitted.
